hufs  (ATCC)

Journal: BMC Cancer

Article Title: Metformin induces ZFP36 by mTORC1 inhibition in cervical cancer-derived cell lines

doi: 10.1186/s12885-024-12555-5

Figure Lengend Snippet: Metformin induces ZFP36 expression in cervical cancer-derived-cell lines. HeLa and CaSki cells were treated with metformin 20mM for 24 hours, then RNA was extracted for analysis by RT-qPCR. RT-qPCR of ZFP36 after metformin treatment in ( A ) HeLa and ( B ) CaSki. Representative Western Blot of ZFP36 and densitometric analysis in ( C ) HeLa and ( D ) CaSki, ( E ) HaCaT and ( F ) Human Uterine Fibroblast (HUF). Results are shown as means ± SD of at least three independent experiments. * p <0.05 **, p <0.01

Article Snippet: Primary Human Uterine Fibroblast (HUF) (PCS-460–010) and human cervical cancer-derived cell lines HeLa (RRID: CVCL_0030) and CaSki (RRID: CVCL_1100) were obtained from ATCC (Manassas, VA, USA).

Techniques: Expressing, Derivative Assay, Quantitative RT-PCR, Western Blot

Journal: International journal of molecular sciences

Article Title: Characterization and Clinical Relevance of Endometrial CAFs: Correlation between Post-Surgery Event and Resistance to Drugs.

doi: 10.3390/ijms24076449

Figure Lengend Snippet: Figure 5. A comparison of NCAF with TCAF in terms of the alterations in genes and expression of protein markers: A comprehensive cancer panel report on all exon coverage on 409 genes involved in some of the most common known cancers shows the alteration of genes in aggressive TCAFs and NCAFs from the same patient compared to HUF cells (A). The pathway-based gene selection profiled the mutational spectrum in known cancer driver genes and drug targets along with signaling cascades, apoptosis genes, DNA repair genes, transcription regulators, inflammatory response genes, and growth factor genes. The panel covered both somatic and germline mutations. Heatmaps depicting the genetic alterations identified in NCAF, TCAF, or HUF samples are presented. The coding regions of 409 cancer-related genes are sequenced. Variants with a significant allele frequency difference between sample groups (n = 3) are summarized. Numbers of significantly altered variants of those genes are plotted. A 3-color scale-based conditionally formatted expression (% of expression by ICC) of CAF markers in aggressive TCAFs, and NCAFs from 29 representative patients sorted on the basis of CAFs with corresponding histologies, grades/stages (as presented with color codes, and numbers) is presented in a heatmap (B).

Article Snippet: The complete medium for HUF cells (Primary Uterine Fibroblasts; Normal, Human (ATCC PCS-460-010) consisted of the components of the growth kit with the Fibroblast Basal Medium-Low Serum (ATCC PCS-201-041), which were added to the basal medium (ATCC PCS-201-030).

Techniques: Comparison, Expressing, Selection